Production and Purification of Alkaline Protease Inhibitors ofStreptomycessp.
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چکیده
منابع مشابه
Isolation, Purification and Characterization of a Thermophilic Alkaline Protease from Bacillus subtilis BP-36
The goal of this research was to isolate and identify the thermostable alkaline protease producing bacteria among several native Iranian microorganisms. At the end of screening program, a Bacillus subtilis BP-36 strain producing thermophilic alkaline protease was isolated from a hot spring in Ardebil province. The target enzyme was purified using a one-step Aqueous two-phase systems (ATPS) prot...
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Background: Alkaline proteases is the important group of enzymes having numerous industrial applications including dairy food formulations. Objectives: The current study deals with the purification and characterization of an alkaline serine protease produced by Geotrichum candidum QAUGC01, isolated from indigenous fermented milk product, Dahi.<br...
متن کاملProduction, Purification and Characterization of an Alkaline Protease from an Alkaliphilic Bacillus Sp
An extracellular serine like alkaline protease was purified and characterized from alkaliphilic Bacillus sp. The enzyme was purified to the homogeneity by a combination of aqueous two-phase system and affinity chromatographic techniques. The apparent molecular weight was estimated as 26-29 kDa based on SDS-PAGE and gel filtration chromatography and the enzyme appeared to be a monomer. The Km an...
متن کاملProduction of alkaline protease from Cellulosimicrobium cellulans
Cellulosimicrobium cellulans is one of the microorganisms that produces a wide variety of yeast cell wall-degrading enzymes, β-1,3-glucanase, protease and chitinase. Dried cells of Saccharomyces cerevisiae were used as carbon and nitrogen source for cell growth and protease production. The medium components KH2PO4, KOH and dried yeast cells showed a significant effect (p<0.05) on the factorial ...
متن کاملProduction of alkaline protease by Pseudomonas aeruginosa.
A highly sensitive and specific radioimmunoassay has been developed for Pseudomonas aeruginosa alkaline protease. Production of alkaline protease was found to be strain variable and medium dependent.
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ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1989
ISSN: 0002-1369
DOI: 10.1080/00021369.1989.10869389